Department of Hematology
Hadassah University Hospital
Ein-Karem, Jerusalem
Professor Ariella Oppenheim was born in Israel in 1936.
She earned her PhD in 1966 from the University of Southern California, Davis.
She returned to Israel in 1966 where she became a researcher at the Hebrew University.
In 1976-1977, she was a visiting scientist at the laboratory of Dr. Bob Martin at the National Institutes of Health's Laboratory of Molecular Biology.
She returned to the Virology Department of the Hebrew University, and in 1980-81 she was a visiting scientist at the National Cancer Institute in the laboratory of Prof. Max Gottesman.
Since 1982, she has been affiliated with the Hematology Department of Hadassah University Hospital, where she has received the appointment of Professor of Experimental Hematology.
Prof. Oppenheim has, and continues to, actively supervise a total of dozens of Hebrew University graduate students in studies of Genetics and Biotechnology. Her research projects include both clinical studies and basic science research.
Her clinical projects include the molecular diagnosis of thalassemia and other globin gene disorders.
Prof. Oppenheim's expertise in molecular biology and genetics served her well in establishing the National Laboratory for Thalassemia Diagnosis. This laboratory has made a significant contribution to health care in Israel by performing prenatal diagnosis (about 40 per year) for the severe hereditary anemia known as beta thalassemia.
Prof. Oppenheim has also made significant progress in establishing a gene therapy method which would be safe and clinically applicable, using a novel method which she invented, based on the virus SV40. Her reputation is such that a chair of Gene Therapy has been established in her honor.
In addition, Prof Oppenheim has performed fascinating studies of DNA extracted from archeological remains, which reveal many unexpected facts about the lives of ancient peoples living in this part of the world.
Prof. Oppenheim has made significant contributions to public health in Israel, as well as to the world of science and remains an invaluable member of the hematology department.
Research Subjects
1. Development of somatic gene therapy: We have developed an innovative SV40 pseudoviral vector, which can be produced in the test tube from recombinant viral capsid proteins and purified DNA. Our research is conducted along two main lines: Improvement of the generic vector, in collaboration with Amos Oppenheim, and the development of specific vectors for various target diseases, in collaboration with many investigators, at Hadassah and elsewhere.
2. SV40 virus assembly: During our studies on SV40 assembly we have discovered the SV40 packaging signal and formulated a working model for its role in regulating the viral life cycle and its assembly. We have found that transcription factor Sp1 participate in SV40 assembly by recruiting the capsid proteins to the viral minichromosome. This is the first example of the participation of a transcription factor in virus assembly. These studies led to the development of the in vitro packaging system for SV40 pseudovirions for gene therapy described above.
3. Search for genetic modifiers of globin gene expression: In collaboration with Prof. Deborah Rund and Dr. Dvora Filon we have accomplished a comprehensive characterization of the genetic lesions underlying alpha- and beta-thalassemia in Israel, including the discovery of many novel mutations in alpha- and beta-globin genes. Since then our studies indicated the existence of additional genes which affect the clinical condition of patients and carriers. These additional genes are under investigation. This research provides the scientific basis for molecular diagnosis of thalassemia performed in our diagnostic laboratory in the Department of Hematology.
4. Genetic diversity of Israeli ethnic groups: In collaboration with Dr. Marina Faerman and Dr. Dvora Filon we study mutational diversity in disease-causing genes as well as neutral polymorphic markers, in particular of the Y-chromosome. This research sheds light on questions such as gene flow and mechanisms underlying the evolution of genetic diseases.
5. Genetics of past populations of Israel: In collaboration with Patricia Smith and Marina Faerman we have started to investigate the genetics of past populations using ancient DNA technology. These studies will clarify the origins of ancient communities, their genetic interrelations and their affinities to present day populations.
List of Publications
Oppenheim, Ariella. An acetaldehyde dehydrogenase from higher plants. Ph.D. Dissertation. University of California, Davis, 1966.
1. Castelfranco, Paul, Ariella Oppenheim and Shogo Yamaguchi. Riboflavin-mediated photodecomposition of Amitrole in relation to chlorosis. Weeds 11:11-115, 1963.
2. Liu, Tin-Yin, Ariella Oppenheim and Paul Castelfranco. Ethyl alcohol metabolism in leguminous seedlings. Plants Physiol. 40:1261-1268, 1965.
3. Oppenheim, Ariella and Paul Castelfranco. An acetaldehyde dehydrogenase from germinating seeds. Plant Physiol. 42: 125-132, 1967.
4. Oppenheim, Ariella and Nurit Katzir. Advancing the onset of mitosis by cell free preparations of Physarum polycephalum. Exptl. Cell Research 68:226-244, 1971.
5. Oppenheim, Ariella and Jacob Wahrman. DNA-membrane association during the mitotic cycle of Physarum polycephalum. Exptl. Cell Research 79: 287-294, 1973.
6. Echols, H., L. Green, Amos B. Oppenheim, Ariella Oppenheim and Alik Honigman. The role of the cro gene in bacteriophage lambda development. J. Mol. Biol. 80:203-216, 1973.
7. Oppenheim, Ariella, Alik Honigman and Amos B. Oppenheim. Interference with phage lambda cro function by a colicine-tolerant Escherichia coli mutant. Virology 61:1-10, 1974.
8. Honigman, Alik, Ariella Oppenheim, Amos B. Oppenheim and Wim S. Stevens. A pleiotropic regulatory mutation in lambda bacteriophage. Molec. Gen. Genet. 138:85-111, 1975.
9. Katzir, Nurit, Ariella Oppenheim, Marlene Belfort and Amos B. Oppenheim. Activation of the lambda int gene by the cII and cIII gene products. Virology 74: 324-331, 1976.
10. Oppenheim, Ariella and Amos B. Oppenheim. Suppressible mutations in the cro gene of bacteriophage lambda. Virology 75:469-476, 1976.
11. Oppenheim, Ariella. Suppression of prm mutant by the sar mutation for the synthesis of repressor by bacteriophage lambda. J. Mol. Biol. 111:83-89, 1977.
12. Oppenheim, Amos B., Nurit Katzir and Ariella Oppenheim. Regulation of protein synthesis in bacteriophage lambda: NlRestoration of gene expression in ≠†strains by mutation in the cro gene. Virology 79:405-425, 1977.
13. Oppenheim, Ariella, Marlene Belfort, Nurit Katzir, Nava Kass and Amos B. Oppenheim. Interaction of cII, cIII, and cro gene products in the regulation of early and late functions of phage lambda. Virology 79:426-436, 1977.
14. Oppenheim, Ariella, Nurit Katzir and Amos B. Oppenheim. The product of gene P of coliphage lambda. Virology 79:437-441, 1977.
15. Belfort, Marlene, Nava Kass, Ariella Oppenheim, Nurit Katzir and Amos B. Oppenheim. Repressor and Int synthesis of bacteriophage lambda in the E. coli host mutant ER437. Molec. Gen. Genet. 155:347-349, 1977.
16. Martin, Robert G. and Ariella Oppenheim. Initiation points for DNA replication in nontransformed and simian virus 40-transformed Chinese hamster lung cells. Cell 11:859-869, 1977.
17. Oppenheim, Ariella and Robert G. Martin. Initiation points for DNA replication in nontransformed and simian virus 40-transformed BALB/c 3T3 cells. J. Virol. 25:450-452, 1978.
18. Oppenheim, Ariella and Amos B. Oppenheim. Regulation of the int gene of bacteriophage lambda: Activation by the cII and cIII gene products and the role of the PI and PL promoters. Molec. Gen. Genet. 165:39-46, 1978.
19. Katzir, Nurit, Ariella Oppenheim and Amos B. Oppenheim. Coordinated regulation of the cI and int genes in coliphage lambda and specificity of the cII/cIII activators. Virology 104:398-406, 1980.
20. Oppenheim, Ariella, Ziporah Shlomai and Hannah Ben-Bassat. Initiation points for cellular deoxyribonucleic acid replication in human lymphoid cells converted by Epstein-Barr virus. Mol. Cell. Biol. 1:753-762, 1981.
21. Oppenheim, Ariella and Aviva T. Horowitz. No activation of new initiation points for deoxyribonucleic acid replication in BALB/c 3T3 cells transformed by Kirsten sarcoma virus. Mol. Cell. Biol. 1:763-768, 1981.
22. Oppenheim, Ariella. Separation of closed circular DNA from linear DNA by electrophoresis in two dimensions in agarose gels. Nucl. Acids Res. 9∫6804-6812¨†1981.
23. Oppenheim, Ariella, Yael Katzir, Eitan Fibach, Ada Goldfarb, and Eliezer A. Rachmilewitz. Hypomethylation of DNA derived from purified human erythroid cells correlates with gene activity of the beta-globin cluster. Blood 66:1202-1207,†1985.
24. Oppenheim, Ariella, Aviva Peleg, Eitan Fibach and Eliezer A. Rachmilewitz. Efficient introduction of plasmid DNA into human hemopoietic cells by encapsidation as SV40 pseudovirions. Proc. Natl. Acad. Sci. USA 83:6925-6929, 1986.
25. Oppenheim, Ariella, Alice Karsai, Richard Treisman, Eitan Fibach, Aliza Treves, Ada Goldfarb, Thomas Maniatis, Eliezer A. Rachmilewitz and Gad Glaser. Beta-thalassemia: Analysis of mRNA precursors of a mutant human globin gene with defective splicing using peripheral blood nucleated RBC. Hemoglobin 10: 573-586, 1986.
26. Shalev, Oded, Alice L. Boylen, Cyril Levine, Ariella Oppenheim and Eliezer A. Rachmilewitz. Sickle cell trait in a white Jewish family presenting as splenic infarction at high altitude. Am. J. Hematol. 27:46-48, 1988.
27. Oppenheim, Ariella, Sason Cohen, Ada Goldfarb, Joshua Katzhandler, Joseph Deutsch and Eliezer A. Rachmilewitz. Detection of specific beta-globin mutations in Kurdish Jews with beta-thalassemia. Hemoglobin 12:31-38, 1988.
28. Hopmeier, Pierre, Amir Shenhav, Gad Glaser, Eliezer A. Rachmilewitz and Ariella Oppenheim. A patient of German ©bddescent with (o-thalassemia carrying the Sicilian type deletion of the beta- and delta- globin genes. Hemoglobin 12:39-51, 1988.
29. Fibach, Eitan, Daphna Manor, Ariella Oppenheim and Eliezer A. Rachmilewitz. Proliferation and maturation of human erythroid progenitors in liquid culture. Blood 73:100-103, 1989.
30. Oppenheim, Ariella and Aviva Peleg. Helpers for efficient packaging of SV40 pseudovirions. Gene 77:79-86, 1989.
31. Rund, Deborah, Naomi Kornhendler, Oded Shalev and Ariella Oppenheim. The origin of sickle cell alleles in Israel. Hum. Genet. 85:521-524, 1990.
32. Oppenheim, Ariella, Avi Yaari, Deborah Rund, Eliezer Rachmilewitz, David Nathan, Corinne Wong, Haig H. Kazazian, Jr. and Barbara Miller. Intrinsic potential for high fetal hemoglobin -thalassemia is due to an unlinked geneticbproduction in a Druze family with determinant. Hum. Genet. 86: 175-180, 1990.
33. Kurdi-Haidar, Buran, Philip Mason, Alain Berrebi, George Ankra-Badu, Amin Al-Ali, Ariella Oppenheim and Lucio Luzzatto. Origin and Spread of the Glucose-6-Phosphate Dehydrogenase Variant (G6PD-Mediterranean) in the Middle East. Am. J. Hum. Genet. 47:1013-1019, 1990.
34. Rund, Deborah, Tirza Cohen, Dvora Filon, Carol Dowling, Tina Warren, Igal Barak, Eliezer A. Rachmilewitz, Haig H. Kazazian and Ariella Oppenheim. Evolution of a genetic disease in an ethnic isolate: -Thalassemia in theb Jews of Kurdistan. Proc. Natl. Acad. Sci. USA 88:310-314, 1991.
35. Rund, Deborah, Dvora Filon, Menachem Granat, Yemina Hemo, Tirza Cohen, Ariella Oppenheim and Eliezer A. Rachmilewitz. Prenatal diagnosis of thalassemia: Identification of mutations in conjunction with gene amplification in vitro. Harefuah 120:57-60, 1991 (Hebrew).
36. Rund, Deborah, Dvora Filon, Nurith Strauss, Eliezer A. Rachmilewitz, and Ariella Oppenheim. Mean corpuscular -thalassemia correlates with the severity ofbvolume (MCV) of heterozygotes for mutations. Blood 79:238-243, 1992.
37. Rund, Deborah, Carol Dowling, Khamis Najjar, Eliezer A. Rachmilewitz, Haig H. Kazazian, Jr. and Ariella Oppenheim. Two mutations in the -globin polyadenylylation signal reveal extended transcripts and new RNAb polyadenylylation sites. Proc. Natl. Acad. Sci. USA 89:4324-4328, 1992.
38. Oppenheim, Ariella, Ziv Sandalon, Aviva Peleg, Orly Shaul, Silvia Nicolis and Sergio Ottolenghi. A cis Acting DNA signal for encapsidation of simian Virus 40. J. Virol. 66:5320-5328, 1992.
39. Dalyot, Nava, Eitan Fibach, Eliezer A. Rachmilewitz and Ariella Oppenheim. Adult and neonatal patterns of human globin gene expression are recapitulated in liquid cultures. Exp. Hematol. 20:1141-1145, 1992.
40. Oppenheim, Ariella, Corrine L. Jury, Deborah Rund,Tom J. Vulliamy and Lucio Luzzatto. G6PD Mediterranean accounts for the high prevalence of G6PD deficiency in Kurdish Jews. Human Genetics 91:293-294, 1993.
41. Rund, Deborah, Dvorah Filon, -thalassemia due to abAriella Oppenheim and Ayala Abramov. Silent Carrier -globin mutation interacting with other genetic elements. Eur. J.bsevere Pediatr. 152:574-576, 1993.
42. Oppenheim, Ariella, Varda Oron, Dvorah Filon, Clare C. Fearon, Haig H. Kazazian, Jr. and Deborah Rund. Sporadic -thalassemia in Ashkenaziballeles, including a novel mutation, characterize Jews. Human Mutation 2:152-154, 1993.
43. Dalyot, Nava, Eitan Fibach, Antonella Ronchi, Eliezer A. Rachmilewitz, Sergio Ottolenghi and Ariella Oppenheim. Erythropoietin triggers a burst of GATA-1 in human erythroid differentiation. Nucl. Acids Res. 21:4031-4037, 1993.
44. Oron, Varda, Dvora Filon, Ariella Oppenheim and Deborah Rund. Severe thalassemia intermedia caused by -globin gene triplication with heterozygosityainteraction of homozygosity for thalassemia. British J. Haematol. 86:377-399, 1994.bfor
45. Filon, Dvora, Varda Oron, Svetlana Krichevski, Avraham Shaag, Yechezkel Shaag¨ Tina C. Warren, Ada Goldfarb, Yona Shneor, Ariel Koren, Mehmet Akker, Ayala Abramov, Eliezer A. Rachmilewitz, Deborah Rund, -globin mutations inbHaig H. Kazazian, Jr. and Ariella Oppenheim. Diversity of Israeli ethnic groups reflects recent historic events. Am. J. Hum. Genet. 54:836-843, 1994.
46. Oppenheim, Ariella, Merav Siani, Ziv Sandalon and Galina Mengeritsky. Dynamics of the higher order nucleoprotein structure of simian virus 40 regulatory region during viral development. J. Mol. Biol. 238:501-513, 1994.
47. Oppenheim, Ariella, Dvora Filon, Deborah Rund and Haig Kazazian. Reply to Labie et al. Am. J. Hum. Genet. 55:1285, 1994.
48. Bacon, E. Rena, Nava Dalyot, Dvora Filon, Letizia Schreiber, Eliezer A. Rachmilewitz and Ariella Oppenheim. Hemoglobin switching in humans is accompanied by changes in the ratio of the transcription factors GATA-1 and SP1. Molecular Medicine 1:297-305, 1995.
49. Filon, Dvora, Marina Faerman, thalassemia-b Patricia Smith and Ariella Oppenheim. Sequence analysis reveals a mutation in the DNA of skeletal remains from the archaeological site of Akhziv, Israel. Nature Genetics 9:365-368, 1995.
50. Filon, Dvora, Varda Oron-Karni, Elham Elborno, Theodore Tulchinsky, Deborah Rund, Eliezer Rachmilewitz, Khamis -thalassemia mutationsb Najjar, Ragda Shawa and Ariella Oppenheim. Spectrum of in the Gaza area. Human Mutation 5:351-353, 1995.
51. Filon Dvora, Varda Oron, Yona Shneor, Deborah Rund and Ariella Oppenheim. A frameshift mutation in codon 10 of -globin gene. Human Mutation 6:278, 1995.bthe
52 Faerman, Marina, Dvora Filon, Patricia Smith, Gila Kahila, Charles Greenblatt and Ariella Oppenheim. Sex identification of archaeological human remains based on amplification of the X and Y amelogenin alleles. Gene 167:327-332, 1995.
53. Kapelushnik, Joseph, Reuven Or, Dvora Filon, Arnon Nagler, Gabriel Chividalli, Mehmet Akker, Ella Naparstek, -globin mutations revealsbShimon Slavin and Ariella Oppenheim. Analysis of stable mixed chimerism in thalassemia patients post bone marrow transplantation. Blood 86:3241-3246, 1995.
54. Pugatsch, Thea, Ariella Oppenheim and Shimon Slavin. Improved single-step PCR-assay for sex identification post allogeneic sex-mismatched BMT. Bone Marrow Transplantation 17:273-275, 1996.
55. Or, Reuven, Joseph Kapelushnik, Ella Naparstek, Arnon Nagler, Dvora Filon, Ariella Oppenheim, A. Amar, Mehmet Akker, Simcha Samuel and Shimon Slavin. Second transplantation using allogeneic thalassemia major patient featuring stable-bperipheral blood stem cells in a mixed chimaerism. British J. Haematol. 94:285-287, 1996.
56. Dalyot, Nava, Orly Shaul, Ariela Gordon-Shaag and Ariella Oppenheim, The SV40 packaging signal ses is composed of redundant DNA elements which are partly interchangeable. J. Mol. Biol. 259:69-80, 1996.
57. Oron-Karni, Varda, Dvora Filon, Deborah rund, Eliezer Rachmilewitz and Ariella Oppenheim. Prenatal diagnosis -globin genes. American J.b and -abased on simultaneous DNA analysis for Hematol. 53:203-204,1996
58. Rund, Deborah, Varda Oron-Karni, Dvora Filon, Ada Goldfarb, Eliezer Rachmilewitz and -thalassemia intermedia inbAriella Oppenheim. Genetic analysis of Israel: Diversity of mechanisms and unpredictability of phenotype. American J. Hematol. 54:16-22,1997.
59. Faerman, Marina, Gila Kahila, Dvora Filon, Charles Greenblatt, Lawrence Stager, Patricia Smith and Ariella Oppenheim. Determining the sex of infanticide victims from the Late Roman era through ancient DNA analysis. Nature 385:212-213,1997.
60. Martino, Tina, Feige Kaplan, Stanley Diamond, Ariella Oppenheim and Charles R. Scriver. Probable identity by descent and discovery of familial -thalassemia haplotype. Human Mutationbrelationships by means of a rare 9:86-87, 1997.
61. Sandalon, Ziv, Nava Dalyot-Herman, Amos B. Oppenheim and Ariella Oppenheim. In vitro assembly of SV40 virions and pseudovirions: vector development for gene therapy. Human Gene Therapy, 8:843-849,1997.
62. Oron-Karni, Varda, Dvora Filon, Deborah Rund and Ariella Oppenheim. A novel mechanism generating short deletion/insertions following slippage is suggested by a mutation in the human -globin gene. Hum. Molec. Genet. 6:881-885, 1997.2a
63. Sandalon, Ziv, and Ariella Oppenheim. Self-assembly and protein-protein interactions between the SV40 capsid proteins produced in insect cells. Virology 237:414-421, 1997.
64. Gordon-Shaag, Ariela, Orly Ben-Nun-Shaul, Harumi Kasamatsu, Amos B. Oppenheim and Ariella Oppenheim. The SV40 capsid protein VP3 cooperates with the cellular transcription factor Sp1 in DNA-binding and in regulating viral promoter activity. J. Mol. Biol. 275:187-195, 1998.
65. Rund, Deborah, Michal Dagan, Nava Dalyot, Chava Kimchi-Sarfati, Pat Schoenlein, Michael Gottesman and Ariella Oppenheim. Efficient transduction of human hematopoietic cells with the human multidrug resistance gene 1 (MDR1) via SV40 pseudovirions. Human Gene Therapy 9:649-657, 1998.
66. Oron-Karni, Varda, Dvora Filon, Ariella Oppenheim and Deborah Rund. Rapid detection of the common Mediterranean -globin deletion/rearrangements using PCR. Amer. J. Hematol. 58:306-310,a 1998.
67. Faerman, Marina, Gila Kahila, Dvora Filon, Charles Greenblatt, Lawrence Stager, Ariella Oppenheim and Patricia Smith. Determining the sex of infanticide victims from the Late Roman era through ancient DNA analysis. J Archaeological Science 25:861-865, 1998.
68. Dalyot-Herman, Nava, Deborah -globin in erythroid progenitors ofbRund and Ariella Oppenheim. Expression of -thalassemia patients using an SV40-based gene delivery system. J.b Hematotherapy & Stem Cell Res. 8:593-599, 1999.
69. Faerman, Marina, Almut Nebel, Dvora Filon, Mark G. Thomas, Neil Bradman, Bruce D. Ragsdale, Michael Schultz and Ariella Oppenheim. From a dry bone to a genetic portrait: A case study of sickle cell anemia. Am J Phys. Anthropol. 111:153-163, 2000.
70. Hammer MF, Redd AJ, Wood ET, Bonner MR, Jarjanazi H, Karafet T, Santachiara-Benerecetti S, Oppenheim A, Jobling MA, Jenkins T, Ostrer H, Bonne-Tamir B. Jewish and Middle Eastern non-Jewish populations share a common pool of Y-chromosome biallelic haplotypes. Proc Natl Acad Sci U. S. A., 97:6769-74, 2000.
71. Filon, Dvora, Ariella Oppenheim, Eliezer A. Rachmilewitch, Rafi Kot and -thalassemia in Vietnam. Hemoglobin,bDoung Ba Truc. Molecular analysis of 24:99-104, 2000.
72. Oron-Krni, Varda, Dvora Filon, Yulia shifrin, Elchanan Fried, Glina Pogrebijsky, Ariella Oppenheim and Deborah -globin mutations and clinical presentation ofaRund. Diversity of -thalassemia.ina. Israel. Amer. J. Hematol. 65:196-203, 2000.
73. Richards M; Macaulay V; Hickey E; Vega E; Sykes B Guida V; Rengo C; Sellitto D; Cruciani F; Kivisild T Villems R; Thomas M; Rychkov S; Rychkov O; Rychkov Y; Golge M Dimitrov D; Hill E; Bradley D; Romano V; Cali F; Vona G Demaine A; Papiha S; Triantaphyllidis C; Stefanescu G; Hatina J Belledi M; Di Rienzo A; Oppenheim A; Norby S; Al-Zaheri N Santachiara-Benerecetti S; Scozzari R; Torroni A; Bandelt HJ. Tracing European Founder Lineages in the Near Eastern mtDNA Pool. Amer. J. Hum. Genet. 67:1251-1276 (2000).
74. Nebel, Almut, Dvora Filon, Deborah A. Weiss, Michael Weale, Marina Faerman, Ariella Oppenheim, Mark G. Thomas. High-resolution Y chromosome haplotypes of Israeli and Palestinian Arabs reveal geographic substructure and substantial overlap with haplotypes of Jews. Human Genetics 107:630-641 (2000).
75. Nebel, Almut, Dvora Filon, Carsten Hohoff, Marina Faerman, Bernd Brinkmann and Ariella Oppenheim. Haplogroup-specific deviation from the stepwise mutation model at the microsatellite loci DYS388 and DYS392. Eur. J. Hum. Genet. 9:22-26 (2001).
76. Wrobel, Borys, Yael Yosef, Amos B. Oppenheim and Ariella Oppenheim. Production and purification of SV40 major capsid protein (VP1) in Eschericia coli strains deficient for the GroELS chaperone machine. Biotechnology 84, 285-289 (2001).
77. Mukherjee, Namita, Almut Nebel, Ariella Oppenhein and Partha P. Majumder. High resolution mapping of Y-chromosomal polymorphisms reveals signatures of population movements from central and west Asia into India. J. Genet., 80:125-135 (2001).
78. Nebel, Almut, Dvora Filon, Bernd Brinkmann, Partha P. Majumder, Marina Faerman, Ariella Oppenheim. The Y chromosome pool of Jews as part of the genetic landscape of the Middle East. Amer. J. Hum. Genet. Am. J. Hum. Genet. 69:1095-1112 (2001).
79. Kimchi-Sarfaty, Chava, Orly Ben-Nun-Shaul, Deborah Rund, Ariella Oppenheim, and Michael M. Gottesman.. In Vitro-Packaged SV40 Pseudovirions as Highly Efficient Vectors for Gene Transfer. Human Gene Therapy 13:299-310 (2002).
80. Abd El-latif, Mahmoud, Dvora globin mutation IVS1-6 accounts-bfilon, Ariella Oppenheim and Moien Kanaan. The for half of the thalassemia chromosomes in the Palestinian population of the mountain region. Hemoglobin, 26:33-40 (2002).
81. Norkin, Leonard C., Howard A. Anderson, Scott A. Wolfrom, and Ariella Oppenheim. Caveolae-mediated simian virus 40 entry is followed by brefeldin A-sensitive transport to the endoplasmic reticulum where the virus disassembles. J. Virol. 76:5156-5166 (2002).
82. Gordon-Shaag, Ariela, Orly Ben-Nun-Shaul, Vered Roitman, Yael Yosef and Ariella Oppenheim. Cellular Transcription Factor Sp1 Recruits the SV40 Capsid Proteins to the Viral Packaging Signal ses. J. Virol. 76:5915-5924 (2002).
83. Nebel , Almut, Ella Landau-Tasseron, Dvora Filon, Ariella Oppenheim, and Marina Faerman. Genetic evidence for the expansion of Arabian tribes into the Southern Levant and North Africa Am. J. Hum. Genet. 70:1594-1596 (2002).
84. Arad, Uri, Orly Ben-Nun-Shaul, Mahmoud Abd El-Latif, Orna Nissim and Ariella Oppenheim. A new packaging cell-line for SV40 vectors that eliminates the generation of T-antigen positive, replication competent recombinants. Virology, 340:155-159 (2002).
85. Koren, Ariel, Lucia Zalman, Haia Palmor, Edna Ekstein, Yona Schneour, Stavit, Shalev, Eliezer A. Rachmilewitz, Dvora Filon, Ariella Oppenheim. The prevention program for beta-thalassemia in the Jezreel and Eiron valleys: results of fifteen years experience. Harefuah 141:938-943 (2002).
86. Chava Kimchi-Sarfaty, Mili Arora, Ariella Oppenheim, and Michael M Gottesman. High cloning capacity of in vitro packaged SV40 vectors with no SV40 virus sequences. Human Gene Therapy, in press.
Approved Patents
Ariella Oppenheim, Nava Dalyot, Orly Ben-Nun-Shaul, Deborah Rund, Ziv Sandalon, Tova Chajek-Shaul and Shulamit Metzger. SV40-derived DNA constructs comprising exogenous DNA sequences. US Patent #6,090,608, 18.7.2000.
Ariella Oppenheim, Ziv Sandalon and Amos Oppenheim. In vitro construction of SV40 viruses and pseudoviruses. Australian Patent #719601, 11. 5.2000.
Patent Applications
Ariella Oppenheim and Nava Dalyot. SV40-derived DNA constructs comprising exogenous DNA sequences. Israeli patent application No. 109558, May 4 1994.
Ariella Oppenheim, Nava Dalyot, Orly Ben-Nun-Shaul, Deborah Rund, Ziv Sandalon, Tova Chajek-Shaul and Shulamit Metzger. SV40-derived DNA constructs comprising exogenous DNA sequences. International PCT/US95/07519, May 4 1995. Canada, 4.12.96; European Patent Organization 95918395.5, 4.12.96.
Ariella Oppenheim, Ziv Sandalon and Amos Oppenheim. In vitro construction of SV40 viruses and pseudoviruses. Israeli patent application No. 115880, November 6 1995. International PCT/IL96/00143, November 6, 1996. USA 09/068,293, 05.06.98; Australia, 73314/96; Canada; European Patent Organization 96 93 5316.8.
Ariella Oppenheim, Ariela Gordon-Shaag. In vitro assembly of SV40 viruses and pseudoviruses. Israeli patent application No. 136610, June 6, 2000.
Ariella Oppenheim, Uri Arad. A new packaging cell-line for SV40 vectors that eliminates the generation of T-antigen positive, replication competent recombinants. Israeli Application #145463, September 16, 2001. PCT/IL02/00757, September 12, 2002.
Invited Papers and Chapters in Books
1. Oppenheim, Amos B., Alik Honigman, Ariella Oppenheim and Wim S. Stevens. The interaction between bacteriophage lambda and its bacterial host: Regulation of developmental pathways. In: Dynamic Aspects of Host-Parasite relationships, Vol. III, Avivah Zuckerman, ed., John Wiley and Sons, New York, Toronto, Israel University Press, Jerusalem, pp. 40-45, 1975.
2. Martin, Robert G., Maria Persico-Dilauro, C.A.F. Edwards and Ariella Oppenheim. The molecular basis of transformation by SV40: The semi-autonomous replicon revisited. In: Molecular Basis of Host-Virus Interaction. Maharani Chakravorty, ed., International Union of Biochemistry, Vol 78. Science Press, Princeton, N.J., pp. 225-239, 1976.
3. Martin, Robert G., Maria Persico-Dilauro, C.A.F. Edwards and Ariella Oppenheim. The molecular basis of transformation by simian virus 40. In: Genetic manipulation as it affects the cancer problem. D. Schultz and Z. Brada, ed., Miami Winter Symposia, Vol. 14. Academic Press, Inc. New York, pp. 87-100, 1977.
4. Oppenheim, Ariella, Aviva Peleg and Eliezer A. Rachmilewitz. Efficient introduction and transient expression of exogenous genes in human hemopoietic cells. In: Normal and Neoplastic Blood Cells: From Genes to Therapy, Vol. 511, C. Peschle, ed., Annals of the New York Academy of Sciences, New York, pp. 418-427, 1987.
5. Rachmilewitz, Eliezer A., Ariella Oppenheim and Oded Shalev. The red blood cell in thalassemia. In: Genetically Abnormal Red Cells, Vol. I. R.L. Nagel, ed., CRC Press, Inc., pp. 137- 160, 1988.
6. Dalyot, Nava and Ariella Oppenheim. Efficient transfer of the complete human beta-globin gene into human and mouse hemopoietic cells via SV40 pseudovirions. In: Gene Transfer and Gene Therapy, Vol. 87, A.L. Beaudet, R. Mulligan, and I.M. Verma, eds., UCLA Symposia on Molecular and Cellular Biology, Alan R. Liss, Inc., New York, pp. 47-56, 1989.
7. Rund, Deborah, Dvora Filon, Carol Dowling, Haig H. Kazazian, Jr., Eliezer A. Rachmilewitz and Ariella Oppenheim. Molecular analysis of beta-thalassemia in Israel: Mutational analysis and expression studies. Ann. New York Acad. Sci. 612:98-105, 1990.
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