Viral Vector Construction Unit

The Core Laboratory has the capability to create two types of viral vector contructs:

Adenoviral Vector

Recombinant adenoviral vectors are generated by recombination in bacteria according to the method of He et al, described in “A simplified system for generating recombinant adenoviruses.” We provide necessary plasmids, bacteria, and continuous guidance up to the final step of obtaining a lysate of the recombinant adenovirus.

Lentiviral Vector

Hybrid lentiviral particles are generated basically, according to the method described by Follenzi and Naldini, by triple transient transfection of three different plasmids into HEK293T cells. We can provide the three vectors required for this transfection. Of the three vectors, the basic transfer vector, used for cloning the gene of interest, is supplied freely. We possess an advanced, modified, transfer vector for which we require a Material Transfer Agreement (MTA). We provide our improved protocol for the generation of hybrid lentiviral particles, as well as continuous guidance of researchers through the process.

Interested investigators are requested to download and complete the online vector application order form in  Word, and hand deliver or e-mail it to:

HillaGiladi, Ph.D.

The Goldyne Savad Institute of Gene Therapy

Hadassah University Hospital, Ein Kerem

Jerusalem, 91120, Israel

Phone: 02-677-9984

Fax: 02-643-0982

e-mail: giladi@hadassah.org.il

Important: We would appreciate, that any publication arising from the generation of viral construct services acknowledge this service as follows:

“The viral construct services were developed at the Israeli National Knowledge Center for Gene Therapy of the Israeli Ministry of Science, the Goldyne Savad Institute of Gene Therapy, Hadassah University Hospital, Jerusalem, Israel.”