Pharmacogenomic Unit
The Pharmacogenomic Unit of the Israeli National Knowledge Center for Gene Therapy of the Israeli Ministry of Science at Goldyne Savad Institute of Gene Therapy provides assistance in gene expression profiling as follows:
1. Consultations on the gene expression profiling platforms and methods suitable for a specific project.
2. Printing of DNA probes onto nylon membranes 8 x 12 cm (see below).
3. All protocols required for work with macroarrays: production of probes, purification and labeling of RNA, hybridization, and membrane processing.
We provide printing of customer's DNA probes (PCR-fragments, plasmids, etc.) from 96-well plates onto nylon membranes (8 x 12 cm) by the “Biorobotic” array machine. The array's design is flexible, and may be modified to match the customer's requirements. With each gene presented by two spots, the maximal capacity of such membrane is 576 genes. We recommend using the total DNA of the appropriate animal species for signal normalization control, as well as for an orientation grid. Total bacterial DNA may be included as a negative control for hybridization. Total RNA from appropriate samples are radioactively labeled and hybridized with the gene probes on the array. The radioactive signals of the hybridized arrays are scanned by a phosphoimager and processed with the aid of the “VisualGrid” program (GPC-biotech; free accessible on the Internet) or a similar software.
Interested investigators are requested to download and complete the online application Pharmacogenic Unit order form in Word, and hand deliver or e-mail it to:
Daniel Goldenberg, Ph.D.
The Goldyne Savad Institute of
Gene Therapy
Hadassah University Hospital, Ein Kerem
Jerusalem, 91120, Israel
Phone: 02-677-8108 (office)
02-677-8781 (lab)
Fax: 02-643-0982
e-mail: goldenberg@hadassah.org.il
Gene probes for cDNA array should be prepared at concentrations of 0.11-1.1 ng/nl in sufficient amount for all ordered membranes (90 nl per one spot; 180 nl per one probe).
The customer has the following obligations:
§ To cover the price of nylon membranes that have been used for the arrays;
§ To provide a short description of the project on a separate page;
§ To acknowledge our service in all publications from the data obtained with these arrays as follows:
“The membrane cDNA array services were developed at the Israeli National Knowledge Center for Gene Therapy of the Israeli Ministry of Science, the Goldyne Savad Institute of Gene Therapy, Hadassah University Hospital, Jerusalem, Israel.”